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Biphenyl 2,3-dioxygenase

natively expressed by: Paraburkholderia xenovorans LB400

Substrates:

2-chlorobiphenyl 2,4-dichlorobiphenyl 2,5-dichlorobiphenyl 3-chlorobiphenyl 3,4-dichlorobiphenyl 4-chlorobiphenyl Bibenzyl biphenyl Biphenylene Cyclohexylbenzene dibenzofuran diphenylacetylene Diphenylmethane flavanone Isopentylbenzene isopropylbenzene n-butyl-benzene trans-Stilbene

Compound	Product	Expression System	Reference
2-chlorobiphenyl		Escherichia coli BL21 pAIA50 or pAIA74	+
Seeger M, Timmis KN, Hofer B. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl Environ Microbiol. 1995 Jul;61(7):2654-8. PMID: 7618878
2,4-dichlorobiphenyl		Escherichia coli BL21 pAIA50 or pAIA74	+
Seeger M, Timmis KN, Hofer B. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl Environ Microbiol. 1995 Jul;61(7):2654-8. PMID: 7618878
2,5-dichlorobiphenyl		Escherichia coli BL21 pAIA50 or pAIA74	+
Seeger M, Timmis KN, Hofer B. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl Environ Microbiol. 1995 Jul;61(7):2654-8. PMID: 7618878
3-chlorobiphenyl		Escherichia coli BL21 pAIA50 or pAIA74	+
Seeger M, Timmis KN, Hofer B. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl Environ Microbiol. 1995 Jul;61(7):2654-8. PMID: 7618878
3,4-dichlorobiphenyl		Escherichia coli BL21 pAIA50 or pAIA74	+
Seeger M, Timmis KN, Hofer B. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl Environ Microbiol. 1995 Jul;61(7):2654-8. PMID: 7618878
4-chlorobiphenyl		Escherichia coli BL21 pAIA50 or pAIA74	+
Seeger M, Timmis KN, Hofer B. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl Environ Microbiol. 1995 Jul;61(7):2654-8. PMID: 7618878
4-chlorobiphenyl	cis-2,3-dihydroxy-1[4-chlorophenyl]-cyclohexa-4,6-diene	Escherichia coli BL21 pAIA743	+
McKay DB, Seeger M, Zielinski M, Hofer B, Timmis KN. Heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products. J Bacteriol. 1997 Mar;179(6):1924-30. PubMed Central PMCID: PMC178915. PMID: 9068637
Bibenzyl		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
biphenyl		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
biphenyl		Escherichia coli BL21 pAIA743	+
Overwin, H., González, M., Standfuß-Gabisch, C., Hofer, B., Wray, V., Reichelt, J., … Méndez, V. (2015). Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements. Microbiology, 161(9), 1844–1856. doi: 10.1099/mic.0.000138
biphenyl		Escherichia coli BL21 pT7-6a	+
Gomez-Gil, L., Kumar, P., Barriault, D., Bolin, J. T., Sylvestre, M., & Eltis, L. D. (2007). Characterization of Biphenyl Dioxygenase of Pandoraea pnomenusa B-356 As a Potent Polychlorinated Biphenyl-Degrading Enzyme. Journal of Bacteriology, 189(15), 5705–5715. doi: 10.1128/JB.01476-06
Biphenylene		Escherichia coli BL21 pAIA743	+
Overwin, H., González, M., Standfuß-Gabisch, C., Hofer, B., Wray, V., Reichelt, J., … Méndez, V. (2015). Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements. Microbiology, 161(9), 1844–1856. doi: 10.1099/mic.0.000138
Biphenylene		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
Cyclohexylbenzene		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
dibenzofuran		Paraburkholderia xenovorans LB400	+
L’Abbée, J.-B., Barriault, D., & Sylvestre, M. (2005). Metabolism of dibenzofuran and dibenzo-p-dioxin by the biphenyl dioxygenase of Burkholderia xenovorans LB400 and Comamonas testosteroni B-356. Applied Microbiology and Biotechnology, 67(4), 506–514. doi: 10.1007/s00253-004-1791-3
diphenylacetylene		Escherichia coli BL21 pAIA743	+
Overwin, H., González, M., Standfuß-Gabisch, C., Hofer, B., Wray, V., Reichelt, J., … Méndez, V. (2015). Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements. Microbiology, 161(9), 1844–1856. doi: 10.1099/mic.0.000138
diphenylacetylene		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
Diphenylmethane		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
flavanone		Escherichia coli DH11S pDB31[LB400-bphFG] + pQE31[bphAE]	+
Pham, T. T. M., Tu, Y., & Sylvestre, M. (2012). Remarkable Ability of Pandoraea pnomenusa B356 Biphenyl Dioxygenase To Metabolize Simple Flavonoids. Applied and Environmental Microbiology, 78(10), 3560–3570. doi: 10.1128/AEM.00225-12
Isopentylbenzene		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945
Isopentylbenzene		Escherichia coli BL21 pAIA743	+
Overwin, H., González, M., Standfuß-Gabisch, C., Hofer, B., Wray, V., Reichelt, J., … Méndez, V. (2015). Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements. Microbiology, 161(9), 1844–1856. doi: 10.1099/mic.0.000138
isopropylbenzene		Escherichia coli BL21 pSHF400	+
Suenaga H, Mitsuoka M, Ura Y, Watanabe T, Furukawa K. Directed evolution of biphenyl dioxygenase: emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes. J Bacteriol. 2001 Sep;183(18):5441-4. PubMed PMID: PMID: 11514531
n-butyl-benzene		Escherichia coli BL21 pSHF400	+
Suenaga H, Mitsuoka M, Ura Y, Watanabe T, Furukawa K. Directed evolution of biphenyl dioxygenase: emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes. J Bacteriol. 2001 Sep;183(18):5441-4. PubMed PMID: PMID: 11514531
trans-Stilbene		Escherichia coli BL21 pAIA743	+
Overwin, H., González, M., Standfuß-Gabisch, C., Hofer, B., Wray, V., Reichelt, J., … Méndez, V. (2015). Permissivity of the biphenyl-specific aerobic bacterial metabolic pathway towards analogues with various steric requirements. Microbiology, 161(9), 1844–1856. doi: 10.1099/mic.0.000138
trans-Stilbene		Escherichia coli BL21 pAIA111 bphA1A2A3A4	+
Gibson DT. Beijerinckia sp strain B a strain by any other name. J Ind Microbiol Biotechnol. 1999 Oct;23(4-5):284-293. PMID: 11423945