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Toluene 4-monooxygenase

natively expressed by: Pseudomonas mendocina KR1

Non-substrates:

1,4-dihydroxyanthraquinone 3-phenylpropyne 4-ethynyltoluene acetylene anthraquinone bromomethane dichloromethane ethyl acetate ethynylbenzene p-aminobenzoate

Compound	Expression System	Inhibitor	Reference
1,4-dihydroxyanthraquinone	Pseudomonas mendocina KR1		+
Oppenheim SF, Studts JM, Fox BG, Dordick JS. Aromatic hydroxylation catalyzed by toluene 4-monooxygenase in organic solvent/aqueous buffer mixtures. Appl Biochem Biotechnol. 2001 Mar;90(3):187-97. PMID: 11318032
3-phenylpropyne	Pseudomonas mendocina KR1	Yes	+
Keener WK, Watwood ME, Schaller KD, Walton MR, Partin JK, Smith WA, Clingenpeel SR. Use of selective inhibitors and chromogenic substrates to differentiate bacteria based on toluene oxygenase activity. J Microbiol Methods. 2001 Sep;46(3):171-85. PMID: 11438182
4-ethynyltoluene	Pseudomonas mendocina KR1	Yes	+
Keener WK, Watwood ME, Schaller KD, Walton MR, Partin JK, Smith WA, Clingenpeel SR. Use of selective inhibitors and chromogenic substrates to differentiate bacteria based on toluene oxygenase activity. J Microbiol Methods. 2001 Sep;46(3):171-85. PMID: 11438182
acetylene	Pseudomonas mendocina KR1	Yes	+
McClay K, Fox BG, Steffan RJ. Chloroform mineralization by toluene-oxidizing bacteria. Appl Environ Microbiol. 1996 Aug;62(8):2716-22. PMID: 8702263
anthraquinone	Pseudomonas mendocina KR1		+
Oppenheim SF, Studts JM, Fox BG, Dordick JS. Aromatic hydroxylation catalyzed by toluene 4-monooxygenase in organic solvent/aqueous buffer mixtures. Appl Biochem Biotechnol. 2001 Mar;90(3):187-97. PMID: 11318032
bromomethane	Pseudomonas mendocina KR1		+
Goodwin KD, Tokarczyk R, Stephens FC, Saltzman ES. Description of toluene inhibition of methyl bromide biodegradation in seawater and isolation of a marine toluene oxidizer that degrades methyl bromide. Appl Environ Microbiol. 2005 Jul;71(7):3495-503. PubMed Central PMCID: PMC1169029. PMID: 16000753
dichloromethane	Pseudomonas mendocina KR1		+
Leahy JG, Tracy KD, Eley MH. Degradation of mixtures of aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbon-degrading bacteria. FEMS Microbiol Ecol. 2003 Mar 1;43(2):271-6. doi: 10.1111/j.1574-6941.2003.tb01067.x. PMID: 19719688
ethyl acetate	Pseudomonas mendocina KR1	Yes	+
Oppenheim SF, Studts JM, Fox BG, Dordick JS. Aromatic hydroxylation catalyzed by toluene 4-monooxygenase in organic solvent/aqueous buffer mixtures. Appl Biochem Biotechnol. 2001 Mar;90(3):187-97. PMID: 11318032
ethynylbenzene	Pseudomonas mendocina KR1	Yes	+
Keener WK, Watwood ME, Schaller KD, Walton MR, Partin JK, Smith WA, Clingenpeel SR. Use of selective inhibitors and chromogenic substrates to differentiate bacteria based on toluene oxygenase activity. J Microbiol Methods. 2001 Sep;46(3):171-85. PMID: 11438182
p-aminobenzoate	Pseudomonas mendocina KR1	Yes	+
Bailey LJ, Acheson JF, McCoy JG, Elsen NL, Phillips GN Jr, Fox BG. Crystallographic analysis of active site contributions to regiospecificity in the diiron enzyme toluene 4-monooxygenase. Biochemistry. 2012 Feb 14;51(6):1101-13. doi: 10.1021/bi2018333. PMID: 22264099