RAPID | for identifying and exploiting enzyme catalysis.

Enzyme	Expression System	Inhibitor	Reference
Naphthalene 1,2-dioxygenase	Escherichia coli JM109 pDTG141	Yes	+
Lee K. Benzene-induced uncoupling of naphthalene dioxygenase activity and enzyme inactivation by production of hydrogen peroxide. J Bacteriol. 1999 May;181(9):2719-25. PubMed Central PMCID: PMC93710. PMID: 10217759
Cytochrome P450(BM-3)	Escherichia coli pCYTEXp1		+
Wong TS, Wu N, Roccatano D, Zacharias M, Schwaneberg U. Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds. J Biomol Screen. 2005 Apr;10(3):246-52. PMID: 15809320
Biphenyl 2,3-dioxygenase	Paraburkholderia xenovorans LB400		+
Haddock JD, Gibson DT. Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400. J Bacteriol. 1995 Oct;177(20):5834-9. Erratum in: J Bacteriol 1996 Apr;178(7):258. PubMed Central PMCID: PMC177406. PMID: 7592331
Biphenyl 2,3-dioxygenase	Escherichia coli BL21 pSHF400		+
Suenaga H, Mitsuoka M, Ura Y, Watanabe T, Furukawa K. Directed evolution of biphenyl dioxygenase: emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes. J Bacteriol. 2001 Sep;183(18):5441-4. PubMed PMID: PMID: 11514531