RAPID | for identifying and exploiting enzyme catalysis.

Substrate	Enzyme	Expression System	Reference
trans-1,4-dichloro-2-butene	Toluene 2,3-dioxygenase	Escherichia coli BL21 pDTG601a todC1C2BA	+
Lange CC, Wackett LP. Oxidation of aliphatic olefins by toluene dioxygenase: enzyme rates and product identification. J Bacteriol. 1997 Jun;179(12):3858-65. PubMed Central PMCID: PMC179193. PMID: 9190800