RAPID | for identifying and exploiting enzyme catalysis.

Substrate	Enzyme	Expression System	Reference
o-cresol	Toluene 4-monooxygenase	Escherichia coli TG1 pBS(Kan)T4MO tmoABCDEF	+
Tao Y, Fishman A, Bentley WE, Wood TK. Altering toluene 4-monooxygenase by active-site engineering for the synthesis of 3-methoxycatechol, methoxyhydroquinone, and methylhydroquinone. J Bacteriol. 2004 Jul;186(14):4705-13. PubMed Central PMCID: PMC438599. PMID: 15231803
o-methoxy phenol	Toluene 4-monooxygenase	Escherichia coli TG1 pBS(Kan)T4MO tmoABCDEF	+
Fishman A, Tao Y, Wood TK. Toluene 3-monooxygenase of Ralstonia pickettii PKO1 is a para-hydroxylating enzyme. J Bacteriol. 2004 May;186(10):3117-23. PubMed Central PMCID: PMC400597. PMID: 15126473
Toluene	Toluene 2,3-dioxygenase	Pseudomonas putida UV4	+
Boyd, D. R., Sharma, N. D., Byrne, B., Hand, M. V., Malone, J. F., Sheldrake, G. N., … Dalton, H. (1998). Enzymatic and chemoenzymatic synthesis and stereochemical assignment of cis-dihydrodiol derivatives of monosubstituted benzenes. Journal of the Chemical Society, Perkin Transactions 1, (12), 1935–1944. doi: 10.1039/A800809D