RAPID | for identifying and exploiting enzyme catalysis.

Product	Enzyme	Expression System	Reference
4-chlorophenol	Toluene 4-monooxygenase	Pseudomonas mendocina KR1	+
Pikus JD, Studts JM, McClay K, Steffan RJ, Fox BG. Changes in the regiospecificity of aromatic hydroxylation produced by active site engineering in the diiron enzyme toluene 4-monooxygenase. Biochemistry. 1997 Aug 5;36(31):9283-9. PMID: 9280437
3-chloro-cis-1,2-dihydroxycyclohexa-3,5-diene	Toluene 2,3-dioxygenase	Pseudomonas putida UV4	+
Boyd, D. R., Sharma, N. D., Byrne, B., Hand, M. V., Malone, J. F., Sheldrake, G. N., … Dalton, H. (1998). Enzymatic and chemoenzymatic synthesis and stereochemical assignment of cis-dihydrodiol derivatives of monosubstituted benzenes. Journal of the Chemical Society, Perkin Transactions 1, (12), 1935–1944. doi: 10.1039/A800809D